tPA Production The assignment should be about 600 words and use APA format. It
assignment should be about 600 words and use APA
format. It should be a well-written paper, not outline or Q&A
format. Be careful with proper use of citations.
Read the attached Datar et al.
which is a case study describing the process economics of the production of tPA
using both eukaryotic cells (CHO) and prokaryotic cells (E. coli). Write
a concise and articulate report using prose about 600 words; not outline
or Q&A format) addressing the following questions:
Mammalian culture is generally believed to be more expensive to
operate than prokaryotic culture. What made this case study unique was the
opposite result. In your opinion, what are the main reasons why
recombinant tPA cost less to manufacture from CHO cells than that
from E. coli?
What are the bottlenecks in production of CHO-tPA and Bacterial
Why was the equipment cost of bacterial tPA much higher than that
What are the lessons you have learned from this case study on tPA
bioprocessing? How can they be applied to current biopharmaceuticals?